flagellin protein, about 20,000 subunits make up one flagellum
function
motility and chemotaxis
product
flagellin protein
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.2|Flagellar proteins][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.6|Other proteins required for efficient pellicle biofilm formation][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.4|Swarming][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]Gene
Coordinates
3,634,987 → 3,635,901
Phenotypes of a mutant
no swarming motility on B medium. [Pubmed|19202088]not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]few hours delay in pellicle development [Pubmed|26122431]mutant has increased fitness in planktonic culture when competed with the wild-type NCIB3610 strain [Pubmed|26122431]The protein
Protein family
bacterial flagellin family (according to Swiss-Prot)Paralogous protein(s)
[protein|5A94391906ABFE70C5BA14E1E75343BC5805D0D9|YvzB] (C-terminal domain of Hag)Structure
[PDB|3A5X] (from ''Salmonella typhimurium'', 42% identity)[SW|Localization]
extracellular (no signal peptide), major component of the secretome [Pubmed|18957862]membrane [Pubmed|18763711]additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]Expression and Regulation
Operons
genes
[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]
description
[Pubmed|2498284]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|2498284], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12730172], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon][protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|19118355], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]regulation
repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12730172]view in new tabOther regulations
[protein|93F328524989597C7D2329B25E665496C9631E87|CsrA]: translation inhibition, [Pubmed|17555441]Biological materials
Mutant
GP901 (aphA3), GP902 (tet) [Pubmed|21856853], both available in [SW|Jörg Stülke]'s lab1A915 ( ''hag''::''cat''), [Pubmed|19202088], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A915&Search=1A915 BGSC]1A783 ( ''hag''::''erm''), [Pubmed|2174860], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A783&Search=1A783 BGSC]1A842 ( ''hag''::''kan''), [Pubmed|14762011], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A842&Search=1A842 BGSC]DS1677 (marker-less in NCIB3610) [Pubmed|18566286]BKE35360 (Δ[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTTGTTCCTCCCTG, downstream forward: _UP4_TAATTTTAAAAAAGACCTTGBKK35360 (Δ[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTTGTTCCTCCCTG, downstream forward: _UP4_TAATTTTAAAAAAGACCTTGExpression vector
expression in ''B. subtilis'', in [SW|pBQ200]: pGP1089, available in [SW|Jörg Stülke]'s lablacZ fusion
pGP1035 (in [protein|search|pAC6]), pGP755 (in [protein|search|pAC7]), there is also a series of promoter deletion variants in [protein|search|pAC6] and [protein|search|pAC7] [Pubmed|21856853], all available in [SW|Jörg Stülke]'s labGFP fusion
BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in [SW|Jörg Stülke]'s labLabs working on this gene/protein
[SW|Daniel Kearns]References
Reviews
22672726,25251856,26490009 Original Publications
18566286,26122431,23144244,21602220,21856853,2498284,9648743,9096206,7708689,19202088,18763711,21895793,10809682,19118355,17555441,20534509,15378759